17979-Obukhov, Alexander

Alexander G. Obukhov, PhD

Associate Professor of Anatomy, Cell Biology & Physiology

Email
aobukhov@iu.edu
Phone
317-274-8078
Address
635 Barnhill Dr., MS 360A

Indianapolis, IN 46202
PubMed:

Bio

Alexander Obukhov studied electrophysiology and neurophysiology at the Bogomolets Institute of Physiology of the Ukrainian National Academy of Sciences in Kyiv, Ukraine, under the guidance of Dr. Oleg Krishtal and earned his PhD degree in 1988. His early research focused on the single-channel properties and pharmacology of ATP-activated cation channels, now known as P2x receptor-channels, which are expressed in dorsal root and nodose ganglion sensory neurons. 

Dr. Obukhov completed his first postdoctoral training also in the laboratory of Dr. Oleg Krishtal. During that time, he focused on the pharmacological properties of voltage-gated calcium channels in hippocampal and Purkinje neurons. He then pursued a second postdoctoral training in the laboratory of Dr. Günter Schultz at the Institute of Pharmacology, Benjamin Franklin University Clinic, Freie Universität Berlin (West Berlin, Germany), where he investigated the regulation mechanisms and the electrophysiological and pharmacological properties of newly cloned TRP channels. 

After moving to the US in 1999, Dr. Obukhov accepted a faculty position in the Department of Physiology and Pharmacology at New Jersey Medical School, University of Medicine and Dentistry of New Jersey. At New Jersey Medical School, he continued his studies of TRP channels. In 2006, Dr. Obukhov joined the faculty of the Indiana University School of Medicine, where his laboratory focuses on elucidating the role of TRPC channels in metabolic syndrome-associated atherosclerosis, using the Ossabaw pig model. He also explores several other topics, including the mechanisms regulating uterine contractility, the mechanisms underlying endothelial dysfunction in a mouse aorta model, traumatic brain injury-associated vascular dysfunction, diabetic neuropathy, catecholamine secretion regulation in adrenal chromaffin cells, and the function of SLC4A11 transporters in the corneal endothelium. 

Dr. Obukhov has served on study sections for the American Heart Association, the National Institutes of Health (NIH), and the Department of Veterans Affairs (VA). Dr. Obukhov is a member of the editorial boards of several scientific journals, including Scientific Reports, Cells, PLOS One, and Frontiers in Physiology. Dr. Obukhov is also a member of the American Heart Association.

 

Honors and Awards
1993 - Awarded an Alexander von Humboldt Research Fellowship to conduct Postdoctoral Research in Germany
1996 - Received two Research Awards from Benjamin Franklin University Clinic, Freie Universität Berlin (West Berlin, Germany) for studies of TRPL and TRPC1 channels -
Obukhov AG, Harteneck C, Zobel A, Harhammer R, Kalkbrenner F, Leopoldt D, Lückhoff A, Nürenberg B, Schultz G. "Activation of TRPL cation channels by Galpha11 subunits." 
Zitt C, Zobel A, Obukhov AG, Harteneck C, Kalkbrener F, Lückhoff A, Schultz G. "Cloning and characterization of human TRPC1."
1999 - Received a Research Award from Benjamin Franklin University Clinic, Freie Universität Berlin (West Berlin, Germany) for studies on TRPC6 and TRPC3 channels -
Hofmann T, Obukhov AG, Schaefer M, Gudermann T, Schultz G. "Direct activation of human TRPC6 and TRPC3 channels by diacylglycerol."
2009 Received two Research Awards at the CTSI-IU School of Medicine Poster competition-Kumar S, Barbosa C, Chakraborty S, Obukhov AG “The role of TRPC channels in regulating neurite length.”
Neeb Z., Obukhov AG, Long X, Sturek M “Store-operated Ca2+ influx in coronary smooth muscle cells predicts coronary atherosclerosis in the metabolic syndrome.”
2010 - American Heart Association Research Administration Volunteer Recognition Award
2012 - Trustee's Teaching Award from the Indiana University School of Medicine  
2016 - American Heart Association Research Administration Volunteer Recognition Award

 
Teaching
Lectured in:
Biomedical Science I. Biochemical Basis of Biological Processes (IN-GRDM-G715)
Cardiovascular, Renal and Respiratory Function in Health and Disease (IN-GRDM-G735)
Gastrointestinal and Endocrine Function in Health and Disease (IN-GRDM-G736)
Foundation of Electrical Signaling and Ion Channels (IN-GRDM-G743)
Foundation of Neuroscience (IN-GRDM-G780)
Molecular Physiology of Ion Transport (IN-GRDM-G761)
Foundation of Clinical Sciences (IN-ANES-B512)
Fundamentals of Health and Disease (IN-IUSM-93XM640)
Human Physiology (IN-PHSL-F503)
Clinical Physiology & Pathophysiology I for Physician Assistants (IN-MPAS-M531)
Clinical Physiology & Pathophysiology II for Physician Assistants (IN-MPAS-532)

Facilitator in:
Endocrine & Reproductive Biology (IN-IUSM-93XM760)
Gastrointestinal and Nutrition (IN-IUSM-93XM740)

Course Director of:

Molecular Physiology of Ion Transport (IN-GRDM-G761)

Course Co-Director of:

Human Physiology (F503)

Indianapolis Site Leader of:

Fundamentals of Health and Disease (IN-IUSM-93XM640)

Key Publications

Saqib U, Pandey M, Vyas A, Patidar P, Hajela S, Ali A, Tiwari M, Sarkar S, Yadav N, Patel S, Shukla D, Lienemann GN, White FA, García-Perdomo HA, Baig MS, Halade GV, Hajela K, Sharma S, Obukhov AG. Lipoxins as Modulators of Diseases. Cells. 2025 Aug 12;14(16):1244.  

Demaree IS, Kumar S, Tennessen K, Hoang QQ, White FA, Obukhov AG. Effects of TRPC1's Lysines on Heteromeric TRPC5-TRPC1 Channel Function.  Cells. 2024 Dec 6;13(23):2019.

Chen X, Yan Y, Liu Z, Yang S, Li W, Wang Z, Wang M, Guo J, Li Z, Zhu W, Yang J, Yin J, Dai Q, Li Y, Wang C, Zhao L, Yang X, Guo X, Leng L, Xu J, Obukhov AG, Cao R, Zhong W. In vitro and in vivo inhibition of the host TRPC4 channel attenuates Zika virus infection. EMBO Mol Med. 2024 Aug;16(8):1817-1839.  

Chen X, Obukhov AG, Weisman GA, Seye CI. Basal ATP release signals through the P2Y2 receptor to maintain the differentiated phenotype of vascular smooth muscle cells. Atherosclerosis. 2024 Aug;395:117613.  

Solanki K, Bezsonov E, Orekhov A, Parihar SP, Vaja S, White FA, Obukhov AG, Baig MS. Effect of reactive oxygen, nitrogen, and sulfur species on signaling pathways in atherosclerosis. Vascul Pharmacol. 2024 Mar;154:107282.   

Solanki K, Kumar A, Khan MS, Karthikeyan S, Atre R, Zhang KYJ, Bezsonov E, Obukhov AG, Baig MS. Novel peptide inhibitors targeting CD40 and CD40L interaction: A potential for atherosclerosis therapy. Curr Res Struct Biol. 2023 Nov 14;6:100110.  

Smith JA, Nguyen T, Davis BC, Lahiri DK, Hato T, Obukhov AG, White FA. Propranolol treatment during repetitive mild traumatic brain injuries induces transcriptomic changes in the bone marrow of mice. Front Neurosci. 2023 Sep 12;17:1219941.  

Nguyen T, Nguyen N, Cochran AG, Smith JA, Al-Juboori M, Brumett A, Saxena S, Talley S, Campbell EM, Obukhov AG, White FA. Repeated closed-head mild traumatic brain injury-induced inflammation is associated with nociceptive sensitization.  J Neuroinflammation. 2023 Aug 27;20(1):196.  

Smith JA, Nguyen T, Karnik S, Davis BC, Al-Juboori MH, Kacena MA, Obukhov AG, White FA. Repeated mild traumatic brain injury in mice elicits long term innate immune cell alterations in blood, spleen, and brain. J Neuroimmunol. 2023 Jul 15;380:578106.  

Zhang W, Wang M, Lv W, White FA, Chen X, Obukhov AG. Long-Term Treatment with Gadopentetic Acid or Gadodiamide Increases TRPC5 Expression and Decreases Adriamycin Nuclear Accumulation in Breast Cancer Cells. Cells. 2023 May 3;12(9):1304.  

Saqib U, Munjuluri S, Sarkar S, Biswas S, Mukherjee O, Satsangi H, Baig MS, Obukhov AG, Hajela K. Transient Receptor Potential Canonical 6 (TRPC6) Channel in the Pathogenesis of Diseases: A Jack of Many Trades. Inflammation. 2023 Aug;46(4):1144-1160.

Ludwig N, Demaree IS, Yamada C, Nusbaum A, Nichols FC, White FA, Movila A, Obukhov AG. Dihydroceramides Derived from Bacteroidetes Species Sensitize TRPV1 Channels. Int J Mol Sci. 2023 Jan 3;24(1):877.  

Munjuluri S, Wilkerson DA, Sooch G, Chen X, White FA, Obukhov AG. Capsaicin and TRPV1 Channels in the Cardiovascular System: The Role of Inflammation. Cells. 2021 Dec 22;11(1):18.  

Chen X, Sooch G, Demaree IS, White FA, Obukhov AG. Transient Receptor Potential Canonical (TRPC) Channels: Then and Now. Cells. 2020 Aug 28;9(9):1983.  

Sharma RK, Stevens BR, Obukhov AG, Grant MB, Oudit GY, Li Q, Richards EM, Pepine CJ, Raizada MK. ACE2 (Angiotensin-Converting Enzyme 2) in Cardiopulmonary Diseases: Ramifications for the Control of SARS-CoV-2. Hypertension. 2020 Sep;76(3):651-661.

Obukhov AG, Stevens BR, Prasad R, Li Calzi S, Boulton ME, Raizada MK, Oudit GY, Grant MB. SARS-CoV-2 Infections and ACE2: Clinical Outcomes Linked With Increased Morbidity and Mortality in Individuals With Diabetes. Diabetes. 2020 Sep;69(9):1875-1886.

Duan Y, Prasad R, Feng D, Beli E, Li Calzi S, Longhini ALF, Lamendella R, Floyd JL, Dupont M, Noothi SK, Sreejit G, Athmanathan B, Wright J, Jensen AR, Oudit GY, Markel TA, Nagareddy PR, Obukhov AG, Grant MB. Bone Marrow-Derived Cells Restore Functional Integrity of the Gut Epithelial and Vascular Barriers in a Model of Diabetes and ACE2 Deficiency. Circ Res. 2019 Nov 8;125(11):969-988.  

Liang KL, Bursova JO, Lam F, Chen X, Obukhov AG. Ex Vivo Method for Assessing the Mouse Reproductive Tract Spontaneous Motility and a MATLAB-based Uterus Motion Tracking Algorithm for Data Analysis. J Vis Exp. 2019 Sep 1;(151).  

Chelvanambi S, Gupta SK, Chen X, Ellis BW, Maier BF, Colbert TM, Kuriakose J, Zorlutuna P, Jolicoeur P, Obukhov AG, Clauss M. HIV-Nef Protein Transfer to Endothelial Cells Requires Rac1 Activation and Leads to Endothelial Dysfunction Implications for Statin Treatment in HIV Patients. Circ Res. 2019 Oct 11;125(9):805-820.   

Chen X, Duan Y, Riley AM, Welch MA, White FA, Grant MB, Obukhov AG. Long-Term Diabetic Microenvironment Augments the Decay Rate of Capsaicin-Induced Currents in Mouse Dorsal Root Ganglion Neurons. Molecules. 2019; 24(4): 775. doi: 10.3390/molecules24040775.

Chen X, Taylor-Nguyen NN, Riley AM, Herring BP, White FA, Obukhov AG. The TRPC6 inhibitor, larixyl acetate, is effective in protecting against traumatic brain injury-induced systemic endothelial dysfunction. J Neuroinflammation. 2019; 16(1): 21. doi: 10.1186/s12974-019-1407-6.

Ma W, Chen X, Cerne R, Syed SK, Ficorilli JV, Cabrera O, Obukhov AG, Efanov AM. Catechol estrogens stimulate insulin secretion in pancreatic β-cells via activation of the transient receptor potential A1 (TRPA1) channel. J Biol Chem. 2019; 294(8): 2935-2946. 

Li S, Hundal KS, Chen X, Choi M, Ogando DG, Obukhov AG, Bonanno JA. R125H, W240S, C386R, and V507I SLC4A11 mutations associated with corneal endothelial dystrophy affect the transporter function but not trafficking in PS120 cells. Exp Eye Res. 2019; 180:86-91. 

Chen X, Liu D, Zhou D, Si Y, Xu D, Stamatkin CW, Ghozayel MK, Ripsch MS, Obukhov AG, White FA, Meroueh SO. Small-molecule CaVα1⋅CaVβ antagonist suppresses neuronal voltage-gated calcium-channel trafficking. Proc Natl Acad Sci U S A. 2018; 115(45): E10566-E10575. 

Chen X, Meroueh M, Mazur G, Rouse E, Hundal KS, Stamatkin CW, Obukhov AG. Phenylephrine, a common cold remedy active ingredient, suppresses uterine contractions through cAMP signalling. Sci Rep. 2018; 8(1): 11666. 

Li W, Chen X, Riley AM, Hiett SC, Temm CJ, Beli E, Long X, Chakraborty S, Alloosh M, White FA, Grant MB, Sturek M, Obukhov AG. Long-term spironolactone treatment reduces coronary TRPC expression, vasoconstriction, and atherosclerosis in metabolic syndrome pigs. Basic Res Cardiol. 2017; 112(5): 54.

Chen X, Li W, Riley AM, Soliman M, Chakraborty S, Stamatkin CW, Obukhov AG. Molecular Determinants of the Sensitivity to Gq/11-Phospholipase C-dependent Gating, Gd3+ Potentiation, and Ca2+ Permeability in the Transient Receptor Potential Canonical Type 5 (TRPC5) Channel. J Biol Chem. 2017; 292(3): 898-911.

Chen X, Li W, Hiett SC, Obukhov AG. Novel Roles for Kv7 Channels in Shaping Histamine-Induced Contractions and Bradykinin-Dependent Relaxations in Pig Coronary Arteries. PLoS One. 2016; 11(2):e0148569.

Zhang W, Ogando DG, Bonanno JA, Obukhov AG. Human SLC4A11 Is a Novel NH3/H+ Co-transporter. J Biol Chem. 2015; 290(27): 16894-16905.

Chen X, Sun W, Gianaris NG, Riley AM, Cummins TR, Fehrenbacher JC, Obukhov AG. Furanocoumarins are a novel class of modulators for the transient receptor potential vanilloid type 1 (TRPV1) channel. J Biol Chem. 2014; 289(14): 9600-9610.

Chakraborty S, Berwick ZC, Bartlett PJ, Kumar S, Thomas AP, Sturek M, Tune JD, Obukhov AG. Bromoenol lactone inhibits voltage-gated Ca2+ and transient receptor potential canonical channels. J Pharmacol Exp Ther. 2011; 339(2): 329-340.

Kumar S, Chakraborty S, Barbosa C, Brustovetsky T, Brustovetsky N, Obukhov AG. Mechanisms controlling neurite outgrowth in a pheochromocytoma cell line: the role of TRPC channels. J Cell Physiol. 2012; 227(4): 1408-1419.

Edwards JM, Neeb ZP, Alloosh MA, Long X, Bratz IN, Peller CR, Byrd JP, Kumar S, Obukhov AG, Sturek M. Exercise training decreases store-operated Ca2+ entry associated with metabolic syndrome and coronary atherosclerosis. Cardiovasc Res. 2010; 85(3): 631-640.

Hu G, Oboukhova EA, Kumar S, Sturek M, Obukhov AG. Canonical transient receptor potential channels expression is elevated in a porcine model of metabolic syndrome. Mol Endocrinol. 2009; 23(5): 689-699.

Obukhov AG, Nowycky MC. A cytosolic residue mediates Mg2+ block and regulates inward current amplitude of a transient receptor potential channel. J Neurosci. 2005; 25(5):1234-1239.

Obukhov AG, Nowycky MC. TRPC5 activation kinetics are modulated by the scaffolding protein ezrin/radixin/moesin-binding phosphoprotein-50 (EBP50). J Cell Physiol. 2004; 201(2): 227-235.

Obukhov AG, Nowycky MC. TRPC4 can be activated by G-protein-coupled receptors and provides sufficient Ca2+ to trigger exocytosis in neuroendocrine cells. J Biol Chem. 2002; 277(18): 16172-16178.

Hofmann T, Obukhov AG, Schaefer M, Harteneck C, Gudermann T, Schultz G. Direct activation of human TRPC6 and TRPC3 channels by diacylglycerol. Nature. 1999; 397(6716): 259-263.

Obukhov AG, Harteneck C, Zobel A, Harhammer R, Kalkbrenner F, Leopoldt D, Lückhoff A, Nürnberg B, Schultz G. Direct activation of trpl cation channels by Galpha11 subunits. EMBO J. 1996; 15(21): 5833-5838.

Krishtal OA, Marchenko SM, Obukhov AG. Cationic channels activated by extracellular ATP in rat sensory neurons. Neuroscience. 1988; 27(3): 995-1000. 

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